Construct: shRNA TRCN0000051678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012387.1-1152s1c1
DNA Barcode:
CTGAAGGAGTTTCCCATCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PADI4 (23569)
Gene Description:
peptidyl arginine deiminase 4
Transcript:
RefSeq NM_012387.1 (NON-CURRENT)
Match location:
Position 1152 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAAGGAGTTTCCCATCAAA
Hairpin Sequence:
5'-CCGG-CTGAAGGAGTTTCCCATCAAA-CTCGAG-TTTGATGGGAAACTCCTTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAAGGAGTTTCCCATCAAACTCGAGTTTGATGGGAAACTCCTTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAAGGAGTTTCCCATCAAACTCGAGTTTGATGGGAAACTCCTTCAG-3'

Other clones with same target sequence:

(none)