Construct: shRNA TRCN0000051709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002428.2-2221s1c1
DNA Barcode:
CGGTGACATCAGTGCTGCCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MMP15 (4324)
Gene Description:
matrix metallopeptidase 15
Transcript:
RefSeq NM_002428.2 (NON-CURRENT)
Match location:
Position 2221 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGACATCAGTGCTGCCTA
Hairpin Sequence:
5'-CCGG-CGGTGACATCAGTGCTGCCTA-CTCGAG-TAGGCAGCACTGATGTCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGACATCAGTGCTGCCTACTCGAGTAGGCAGCACTGATGTCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGACATCAGTGCTGCCTACTCGAGTAGGCAGCACTGATGTCACCG-3'

Other clones with same target sequence:

(none)