Construct: shRNA TRCN0000051721

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014244.1-556s1c1
DNA Barcode:
GAGGAGGAGTTCTTCATCGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAMTS2 (9509)
Gene Description:
ADAM metallopeptidase with thrombospondin type 1 motif 2
Transcript:
RefSeq NM_014244.1 (NON-CURRENT)
Match location:
Position 556 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051721)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAGGAGTTCTTCATCGAA
Hairpin Sequence:
5'-CCGG-GAGGAGGAGTTCTTCATCGAA-CTCGAG-TTCGATGAAGAACTCCTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAGGAGTTCTTCATCGAACTCGAGTTCGATGAAGAACTCCTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAGGAGTTCTTCATCGAACTCGAGTTCGATGAAGAACTCCTCCTC-3'

Other clones with same target sequence:

(none)