Construct: shRNA TRCN0000051756

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002076.2-1182s1c1
DNA Barcode:
GCTGTATGAGTTTGATATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNS (2799)
Gene Description:
glucosamine (N-acetyl)-6-sulfatase
Transcript:
RefSeq NM_002076.2 (NON-CURRENT)
Match location:
Position 1182 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051756)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTATGAGTTTGATATCAA
Hairpin Sequence:
5'-CCGG-GCTGTATGAGTTTGATATCAA-CTCGAG-TTGATATCAAACTCATACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTATGAGTTTGATATCAACTCGAGTTGATATCAAACTCATACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTATGAGTTTGATATCAACTCGAGTTGATATCAAACTCATACAGC-3'

Other clones with same target sequence:

(none)