Construct: shRNA TRCN0000051829

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014014.2-2549s1c1
DNA Barcode:
CCCTGCACATACAGTCATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNRNP200 (23020)
Gene Description:
small nuclear ribonucleoprotein U5 subunit 200
Transcript:
RefSeq NM_014014.2 (NON-CURRENT)
Match location:
Position 2549 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051829)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGCACATACAGTCATCAT
Hairpin Sequence:
5'-CCGG-CCCTGCACATACAGTCATCAT-CTCGAG-ATGATGACTGTATGTGCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGCACATACAGTCATCATCTCGAGATGATGACTGTATGTGCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGCACATACAGTCATCATCTCGAGATGATGACTGTATGTGCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000327751