Construct: shRNA TRCN0000051867

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012137.2-686s1c1
DNA Barcode:
CCTTAAGATCATGCAACAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDAH1 (23576)
Gene Description:
dimethylarginine dimethylaminohydrolase 1
Transcript:
RefSeq NM_012137.2 (NON-CURRENT)
Match location:
Position 686 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051867)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTAAGATCATGCAACAGAT
Hairpin Sequence:
5'-CCGG-CCTTAAGATCATGCAACAGAT-CTCGAG-ATCTGTTGCATGATCTTAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTAAGATCATGCAACAGATCTCGAGATCTGTTGCATGATCTTAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTAAGATCATGCAACAGATCTCGAGATCTGTTGCATGATCTTAAGG-3'

Other clones with same target sequence:

  1. TRCN0000300762