Construct: shRNA TRCN0000051886

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_495964.1-42s1c1
DNA Barcode:
GACGTTTGGAACGCTGGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP11A (23250)
Gene Description:
ATPase phospholipid transporting 11A
Transcript:
RefSeq XM_495964.1 (NON-CURRENT)
Match location:
Position 42 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051886)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGTTTGGAACGCTGGTATT
Hairpin Sequence:
5'-CCGG-GACGTTTGGAACGCTGGTATT-CTCGAG-AATACCAGCGTTCCAAACGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGTTTGGAACGCTGGTATTCTCGAGAATACCAGCGTTCCAAACGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGTTTGGAACGCTGGTATTCTCGAGAATACCAGCGTTCCAAACGTC-3'

Other clones with same target sequence:

(none)