Construct: shRNA TRCN0000051894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018702.1-1718s1c1
DNA Barcode:
CGATCGATATTCGTGCGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADARB2 (105)
Gene Description:
adenosine deaminase RNA specific B2 (inactive)
Transcript:
RefSeq NM_018702.1 (NON-CURRENT)
Match location:
Position 1718 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATCGATATTCGTGCGGTTA
Hairpin Sequence:
5'-CCGG-CGATCGATATTCGTGCGGTTA-CTCGAG-TAACCGCACGAATATCGATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATCGATATTCGTGCGGTTACTCGAGTAACCGCACGAATATCGATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATCGATATTCGTGCGGTTACTCGAGTAACCGCACGAATATCGATCG-3'

Other clones with same target sequence:

(none)