Construct: shRNA TRCN0000051915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024837.2-2246s1c1
DNA Barcode:
GCAGGGAATAATGCTGTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP8B4 (79895)
Gene Description:
ATPase phospholipid transporting 8B4 (putative)
Transcript:
RefSeq NM_024837.2 (NON-CURRENT)
Match location:
Position 2246 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000051915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGGAATAATGCTGTGGAA
Hairpin Sequence:
5'-CCGG-GCAGGGAATAATGCTGTGGAA-CTCGAG-TTCCACAGCATTATTCCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGGAATAATGCTGTGGAACTCGAGTTCCACAGCATTATTCCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGGAATAATGCTGTGGAACTCGAGTTCCACAGCATTATTCCCTGC-3'

Other clones with same target sequence:

(none)