Construct: shRNA TRCN0000052031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014966.2-3416s1c1
DNA Barcode:
CCGATGGCTGACGTATTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHX30 (22907)
Gene Description:
DExH-box helicase 30
Transcript:
RefSeq NM_014966.2 (NON-CURRENT)
Match location:
Position 3416 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGATGGCTGACGTATTTCAT
Hairpin Sequence:
5'-CCGG-CCGATGGCTGACGTATTTCAT-CTCGAG-ATGAAATACGTCAGCCATCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGATGGCTGACGTATTTCATCTCGAGATGAAATACGTCAGCCATCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGATGGCTGACGTATTTCATCTCGAGATGAAATACGTCAGCCATCGG-3'

Other clones with same target sequence:

  1. TRCN0000299893