Construct: shRNA TRCN0000052069

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018995.1-3510s1c1
DNA Barcode:
GCTGGAATACAGTATTACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MOV10L1 (54456)
Gene Description:
Mov10 like RISC complex RNA helicase 1
Transcript:
RefSeq NM_018995.1 (NON-CURRENT)
Match location:
Position 3510 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052069)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAATACAGTATTACAAA
Hairpin Sequence:
5'-CCGG-GCTGGAATACAGTATTACAAA-CTCGAG-TTTGTAATACTGTATTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAATACAGTATTACAAACTCGAGTTTGTAATACTGTATTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAATACAGTATTACAAACTCGAGTTTGTAATACTGTATTCCAGC-3'

Other clones with same target sequence:

(none)