Construct: shRNA TRCN0000052123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145298.3-720s1c1
DNA Barcode:
CGCGTGAAGATTATGGACGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APOBEC3F (200316)
Gene Description:
apolipoprotein B mRNA editing enzyme catalytic subunit 3F
Transcript:
RefSeq NM_145298.3 (NON-CURRENT)
Match location:
Position 720 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGTGAAGATTATGGACGAT
Hairpin Sequence:
5'-CCGG-CGCGTGAAGATTATGGACGAT-CTCGAG-ATCGTCCATAATCTTCACGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGTGAAGATTATGGACGATCTCGAGATCGTCCATAATCTTCACGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGTGAAGATTATGGACGATCTCGAGATCGTCCATAATCTTCACGCG-3'

Other clones with same target sequence:

(none)