Construct: shRNA TRCN0000052294

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002615.3-964s1c1
DNA Barcode:
CGGAAGCATGAGTATCATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERPINF1 (5176)
Gene Description:
serpin family F member 1
Transcript:
RefSeq NM_002615.3 (NON-CURRENT)
Match location:
Position 964 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052294)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAGCATGAGTATCATCTT
Hairpin Sequence:
5'-CCGG-CGGAAGCATGAGTATCATCTT-CTCGAG-AAGATGATACTCATGCTTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAGCATGAGTATCATCTTCTCGAGAAGATGATACTCATGCTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAGCATGAGTATCATCTTCTCGAGAAGATGATACTCATGCTTCCG-3'

Other clones with same target sequence:

  1. TRCN0000290232