Construct: shRNA TRCN0000052303

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004568.4-536s1c1
DNA Barcode:
GCCCAGATACTTTCTTTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERPINB6 (5269)
Gene Description:
serpin family B member 6
Transcript:
RefSeq NM_004568.4 (NON-CURRENT)
Match location:
Position 536 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052303)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAGATACTTTCTTTCAAT
Hairpin Sequence:
5'-CCGG-GCCCAGATACTTTCTTTCAAT-CTCGAG-ATTGAAAGAAAGTATCTGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAGATACTTTCTTTCAATCTCGAGATTGAAAGAAAGTATCTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAGATACTTTCTTTCAATCTCGAGATTGAAAGAAAGTATCTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000290227