Construct: shRNA TRCN0000052357

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005025.1-1052s1c1
DNA Barcode:
GCCTCTCTGATAATAAGGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERPINI1 (5274)
Gene Description:
serpin family I member 1
Transcript:
RefSeq NM_005025.1 (NON-CURRENT)
Match location:
Position 1052 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052357)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTCTGATAATAAGGAGA
Hairpin Sequence:
5'-CCGG-GCCTCTCTGATAATAAGGAGA-CTCGAG-TCTCCTTATTATCAGAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTCTGATAATAAGGAGACTCGAGTCTCCTTATTATCAGAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTCTGATAATAAGGAGACTCGAGTCTCCTTATTATCAGAGAGGC-3'

Other clones with same target sequence:

(none)