Construct: shRNA TRCN0000052416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000362.3-1689s1c1
DNA Barcode:
CATGCTCTCCAATTTCGGTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TIMP3 (7078)
Gene Description:
TIMP metallopeptidase inhibitor 3
Transcript:
RefSeq NM_000362.3 (NON-CURRENT)
Match location:
Position 1689 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGCTCTCCAATTTCGGTTA
Hairpin Sequence:
5'-CCGG-CATGCTCTCCAATTTCGGTTA-CTCGAG-TAACCGAAATTGGAGAGCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGCTCTCCAATTTCGGTTACTCGAGTAACCGAAATTGGAGAGCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGCTCTCCAATTTCGGTTACTCGAGTAACCGAAATTGGAGAGCATG-3'

Other clones with same target sequence:

(none)