Construct: shRNA TRCN0000052611

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_291584.2-571s1c1
DNA Barcode:
CCATGCAAAGTACAACACCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC343474 (343474)
Gene Description:
similar to Phosphorylase B kinase alpha regulatory chain, skeletal muscle isoform (Phosphorylase kinase alpha M subunit)
Transcript:
RefSeq XM_291584.2 (NON-CURRENT)
Match location:
Position 571 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052611)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCAAAGTACAACACCAA
Hairpin Sequence:
5'-CCGG-CCATGCAAAGTACAACACCAA-CTCGAG-TTGGTGTTGTACTTTGCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCAAAGTACAACACCAACTCGAGTTGGTGTTGTACTTTGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCAAAGTACAACACCAACTCGAGTTGGTGTTGTACTTTGCATGG-3'

Other clones with same target sequence:

(none)