Construct: shRNA TRCN0000052627

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199345.1-570s1c1
DNA Barcode:
CCGATGTGGTTCCAAATGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PI4KAP2 (375133)
Gene Description:
phosphatidylinositol 4-kinase alpha pseudogene 2
Transcript:
RefSeq NM_199345.1 (NON-CURRENT)
Match location:
Position 570 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052627)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGATGTGGTTCCAAATGCAA
Hairpin Sequence:
5'-CCGG-CCGATGTGGTTCCAAATGCAA-CTCGAG-TTGCATTTGGAACCACATCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGATGTGGTTCCAAATGCAACTCGAGTTGCATTTGGAACCACATCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGATGTGGTTCCAAATGCAACTCGAGTTGCATTTGGAACCACATCGG-3'

Other clones with same target sequence:

  1. TRCN0000174244