Construct: shRNA TRCN0000052628

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_291015.3-3088s1c1
DNA Barcode:
CCGGACTTCATGGCTCATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIDINS220 (57498)
Gene Description:
kinase D interacting substrate 220
Transcript:
RefSeq XM_291015.3 (NON-CURRENT)
Match location:
Position 3088 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052628)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGACTTCATGGCTCATATT
Hairpin Sequence:
5'-CCGG-CCGGACTTCATGGCTCATATT-CTCGAG-AATATGAGCCATGAAGTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGACTTCATGGCTCATATTCTCGAGAATATGAGCCATGAAGTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGACTTCATGGCTCATATTCTCGAGAATATGAGCCATGAAGTCCGG-3'

Other clones with same target sequence:

  1. TRCN0000299847