Construct: shRNA TRCN0000052638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173468.2-4141s1c1
DNA Barcode:
GCACTGTTTATTCCAGCTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MOB1B (92597)
Gene Description:
MOB kinase activator 1B
Transcript:
RefSeq NM_173468.2 (NON-CURRENT)
Match location:
Position 4141 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGTTTATTCCAGCTATA
Hairpin Sequence:
5'-CCGG-GCACTGTTTATTCCAGCTATA-CTCGAG-TATAGCTGGAATAAACAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGTTTATTCCAGCTATACTCGAGTATAGCTGGAATAAACAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGTTTATTCCAGCTATACTCGAGTATAGCTGGAATAAACAGTGC-3'

Other clones with same target sequence:

(none)