Construct: shRNA TRCN0000052668

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_059399.4-543s1c1
DNA Barcode:
CCAGATTTCATGAACTCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CIB4 (130106)
Gene Description:
calcium and integrin binding family member 4
Transcript:
RefSeq XM_059399.4 (NON-CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052668)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATTTCATGAACTCCTTT
Hairpin Sequence:
5'-CCGG-CCAGATTTCATGAACTCCTTT-CTCGAG-AAAGGAGTTCATGAAATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATTTCATGAACTCCTTTCTCGAGAAAGGAGTTCATGAAATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATTTCATGAACTCCTTTCTCGAGAAAGGAGTTCATGAAATCTGG-3'

Other clones with same target sequence:

(none)