Construct: shRNA TRCN0000052722

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003866.1-2486s1c1
DNA Barcode:
CCTCCTGATTATATTTCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
INPP4B (8821)
Gene Description:
inositol polyphosphate-4-phosphatase type II B
Transcript:
RefSeq NM_003866.1 (NON-CURRENT)
Match location:
Position 2486 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052722)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTGATTATATTTCACAT
Hairpin Sequence:
5'-CCGG-CCTCCTGATTATATTTCACAT-CTCGAG-ATGTGAAATATAATCAGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTGATTATATTTCACATCTCGAGATGTGAAATATAATCAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTGATTATATTTCACATCTCGAGATGTGAAATATAATCAGGAGG-3'

Other clones with same target sequence:

(none)