Construct: shRNA TRCN0000052732

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371933.1-3638s1c1
DNA Barcode:
CCTGAGACTCTAATTTCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP1R9A (55607)
Gene Description:
protein phosphatase 1 regulatory subunit 9A
Transcript:
RefSeq XM_371933.1 (NON-CURRENT)
Match location:
Position 3638 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052732)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGACTCTAATTTCAGAT
Hairpin Sequence:
5'-CCGG-CCTGAGACTCTAATTTCAGAT-CTCGAG-ATCTGAAATTAGAGTCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGACTCTAATTTCAGATCTCGAGATCTGAAATTAGAGTCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGACTCTAATTTCAGATCTCGAGATCTGAAATTAGAGTCTCAGG-3'

Other clones with same target sequence:

(none)