Construct: shRNA TRCN0000052734

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_113641.4-542s1c1
DNA Barcode:
GCTGTTATTTGTGTGGTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLPP4 (196051)
Gene Description:
phospholipid phosphatase 4
Transcript:
RefSeq XM_113641.4 (NON-CURRENT)
Match location:
Position 542 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052734)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTATTTGTGTGGTGAAA
Hairpin Sequence:
5'-CCGG-GCTGTTATTTGTGTGGTGAAA-CTCGAG-TTTCACCACACAAATAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTATTTGTGTGGTGAAACTCGAGTTTCACCACACAAATAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTATTTGTGTGGTGAAACTCGAGTTTCACCACACAAATAACAGC-3'

Other clones with same target sequence:

(none)