Construct: shRNA TRCN0000052780

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_291991.2-2209s1c1
DNA Barcode:
CCTCAAGATGTCGAAGTAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC387870 (387870)
Gene Description:
similar to protein tyrosine phosphatase, receptor type, Q isoform 1 precursor
Transcript:
RefSeq XM_291991.2 (NON-CURRENT)
Match location:
Position 2209 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052780)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAAGATGTCGAAGTAATT
Hairpin Sequence:
5'-CCGG-CCTCAAGATGTCGAAGTAATT-CTCGAG-AATTACTTCGACATCTTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAAGATGTCGAAGTAATTCTCGAGAATTACTTCGACATCTTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAAGATGTCGAAGTAATTCTCGAGAATTACTTCGACATCTTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000174251