Construct: shRNA TRCN0000052815

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_376010.1-580s1c1
DNA Barcode:
CCTTTGGAGTATCGTTCTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPTEP2 (400927)
Gene Description:
TPTE pseudogene 2
Transcript:
RefSeq XM_376010.1 (NON-CURRENT)
Match location:
Position 580 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052815)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTGGAGTATCGTTCTATT
Hairpin Sequence:
5'-CCGG-CCTTTGGAGTATCGTTCTATT-CTCGAG-AATAGAACGATACTCCAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTGGAGTATCGTTCTATTCTCGAGAATAGAACGATACTCCAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTGGAGTATCGTTCTATTCTCGAGAATAGAACGATACTCCAAAGG-3'

Other clones with same target sequence:

(none)