Construct: shRNA TRCN0000052829

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_495953.1-605s1c1
DNA Barcode:
CGAATGAATTGACAACAGCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TPTEps1 (440140)
Gene Description:
TPTE pseudogene 1
Transcript:
RefSeq XM_495953.1 (NON-CURRENT)
Match location:
Position 605 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000052829)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAATGAATTGACAACAGCAA
Hairpin Sequence:
5'-CCGG-CGAATGAATTGACAACAGCAA-CTCGAG-TTGCTGTTGTCAATTCATTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAATGAATTGACAACAGCAACTCGAGTTGCTGTTGTCAATTCATTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAATGAATTGACAACAGCAACTCGAGTTGCTGTTGTCAATTCATTCG-3'

Other clones with same target sequence:

(none)