Construct: shRNA TRCN0000053058

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012232.2-603s1c1
DNA Barcode:
CCGCAACTTTAAAGTCATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAVIN1 (284119)
Gene Description:
caveolae associated protein 1
Transcript:
RefSeq NM_012232.2 (NON-CURRENT)
Match location:
Position 603 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053058)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCAACTTTAAAGTCATGAT
Hairpin Sequence:
5'-CCGG-CCGCAACTTTAAAGTCATGAT-CTCGAG-ATCATGACTTTAAAGTTGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCAACTTTAAAGTCATGATCTCGAGATCATGACTTTAAAGTTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCAACTTTAAAGTCATGATCTCGAGATCATGACTTTAAAGTTGCGG-3'

Other clones with same target sequence:

(none)