Construct: shRNA TRCN0000053069

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006232.2-341s1c1
DNA Barcode:
CTGACCAGTTTGAGTATGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POLR2H (5437)
Gene Description:
RNA polymerase II subunit H
Transcript:
RefSeq NM_006232.2 (NON-CURRENT)
Match location:
Position 341 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053069)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGACCAGTTTGAGTATGTAA
Hairpin Sequence:
5'-CCGG-CTGACCAGTTTGAGTATGTAA-CTCGAG-TTACATACTCAAACTGGTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGACCAGTTTGAGTATGTAACTCGAGTTACATACTCAAACTGGTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGACCAGTTTGAGTATGTAACTCGAGTTACATACTCAAACTGGTCAG-3'

Other clones with same target sequence:

(none)