Construct: shRNA TRCN0000053100

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015535.1-852s1c1
DNA Barcode:
GCACCGTTTCTCTAACTAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPATS2L (26010)
Gene Description:
spermatogenesis associated serine rich 2 like
Transcript:
RefSeq NM_015535.1 (NON-CURRENT)
Match location:
Position 852 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053100)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCGTTTCTCTAACTAGAT
Hairpin Sequence:
5'-CCGG-GCACCGTTTCTCTAACTAGAT-CTCGAG-ATCTAGTTAGAGAAACGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCGTTTCTCTAACTAGATCTCGAGATCTAGTTAGAGAAACGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCGTTTCTCTAACTAGATCTCGAGATCTAGTTAGAGAAACGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000290823