Construct: shRNA TRCN0000053152

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_290809.3-2335s1c1
DNA Barcode:
GCTGTGAACTTGATCTCCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF4B (6875)
Gene Description:
TATA-box binding protein associated factor 4b
Transcript:
RefSeq XM_290809.3 (NON-CURRENT)
Match location:
Position 2335 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053152)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGAACTTGATCTCCCAA
Hairpin Sequence:
5'-CCGG-GCTGTGAACTTGATCTCCCAA-CTCGAG-TTGGGAGATCAAGTTCACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGAACTTGATCTCCCAACTCGAGTTGGGAGATCAAGTTCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGAACTTGATCTCCCAACTCGAGTTGGGAGATCAAGTTCACAGC-3'

Other clones with same target sequence:

  1. TRCN0000174249