Construct: shRNA TRCN0000053254

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002332.1-4954s1c1
DNA Barcode:
GCGAACAAACACACTGGCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRP1 (4035)
Gene Description:
LDL receptor related protein 1
Transcript:
RefSeq NM_002332.1 (NON-CURRENT)
Match location:
Position 4954 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053254)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAACAAACACACTGGCTAA
Hairpin Sequence:
5'-CCGG-GCGAACAAACACACTGGCTAA-CTCGAG-TTAGCCAGTGTGTTTGTTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAACAAACACACTGGCTAACTCGAGTTAGCCAGTGTGTTTGTTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAACAAACACACTGGCTAACTCGAGTTAGCCAGTGTGTTTGTTCGC-3'

Other clones with same target sequence:

(none)