Construct: shRNA TRCN0000053321

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020116.2-1992s1c1
DNA Barcode:
CCAACTTTGGACAGAGTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FSTL5 (56884)
Gene Description:
follistatin like 5
Transcript:
RefSeq NM_020116.2 (NON-CURRENT)
Match location:
Position 1992 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053321)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACTTTGGACAGAGTCCTT
Hairpin Sequence:
5'-CCGG-CCAACTTTGGACAGAGTCCTT-CTCGAG-AAGGACTCTGTCCAAAGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACTTTGGACAGAGTCCTTCTCGAGAAGGACTCTGTCCAAAGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACTTTGGACAGAGTCCTTCTCGAGAAGGACTCTGTCCAAAGTTGG-3'

Other clones with same target sequence:

(none)