Construct: shRNA TRCN0000053329

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032680.2-1376s1c1
DNA Barcode:
GCTGAGAATACCAAGCTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CRACR2A (84766)
Gene Description:
calcium release activated channel regulator 2A
Transcript:
RefSeq NM_032680.2 (NON-CURRENT)
Match location:
Position 1376 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053329)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGAATACCAAGCTGAAA
Hairpin Sequence:
5'-CCGG-GCTGAGAATACCAAGCTGAAA-CTCGAG-TTTCAGCTTGGTATTCTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGAATACCAAGCTGAAACTCGAGTTTCAGCTTGGTATTCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGAATACCAAGCTGAAACTCGAGTTTCAGCTTGGTATTCTCAGC-3'

Other clones with same target sequence:

(none)