Construct: shRNA TRCN0000053412

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022137.2-440s1c1
DNA Barcode:
CTACGAGTCCATGTGTGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMOC1 (64093)
Gene Description:
SPARC related modular calcium binding 1
Transcript:
RefSeq NM_022137.2 (NON-CURRENT)
Match location:
Position 440 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053412)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACGAGTCCATGTGTGAGTA
Hairpin Sequence:
5'-CCGG-CTACGAGTCCATGTGTGAGTA-CTCGAG-TACTCACACATGGACTCGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACGAGTCCATGTGTGAGTACTCGAGTACTCACACATGGACTCGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACGAGTCCATGTGTGAGTACTCGAGTACTCACACATGGACTCGTAG-3'

Other clones with same target sequence:

(none)