Construct: shRNA TRCN0000053508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145231.1-193s1c1
DNA Barcode:
CCTCCAAGATAGAAGTGGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EFCAB11 (90141)
Gene Description:
EF-hand calcium binding domain 11
Transcript:
RefSeq NM_145231.1 (NON-CURRENT)
Match location:
Position 193 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCAAGATAGAAGTGGATT
Hairpin Sequence:
5'-CCGG-CCTCCAAGATAGAAGTGGATT-CTCGAG-AATCCACTTCTATCTTGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCAAGATAGAAGTGGATTCTCGAGAATCCACTTCTATCTTGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCAAGATAGAAGTGGATTCTCGAGAATCCACTTCTATCTTGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000300038