Construct: shRNA TRCN0000053533

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033429.1-885s1c1
DNA Barcode:
GAAGCAGATATCGAACCCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CALML4 (91860)
Gene Description:
calmodulin like 4
Transcript:
RefSeq NM_033429.1 (NON-CURRENT)
Match location:
Position 885 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053533)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCAGATATCGAACCCAAT
Hairpin Sequence:
5'-CCGG-GAAGCAGATATCGAACCCAAT-CTCGAG-ATTGGGTTCGATATCTGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCAGATATCGAACCCAATCTCGAGATTGGGTTCGATATCTGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCAGATATCGAACCCAATCTCGAGATTGGGTTCGATATCTGCTTC-3'

Other clones with same target sequence:

(none)