Construct: shRNA TRCN0000053547

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002380.2-2470s1c1
DNA Barcode:
CCAAGGCCAATGGTATCACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MATN2 (4147)
Gene Description:
matrilin 2
Transcript:
RefSeq NM_002380.2 (NON-CURRENT)
Match location:
Position 2470 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053547)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGCCAATGGTATCACTA
Hairpin Sequence:
5'-CCGG-CCAAGGCCAATGGTATCACTA-CTCGAG-TAGTGATACCATTGGCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGCCAATGGTATCACTACTCGAGTAGTGATACCATTGGCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGCCAATGGTATCACTACTCGAGTAGTGATACCATTGGCCTTGG-3'

Other clones with same target sequence:

(none)