Construct: shRNA TRCN0000053594

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004598.2-1134s1c1
DNA Barcode:
CGGTGTAATGAGGAGGGCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPOCK1 (6695)
Gene Description:
SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1
Transcript:
RefSeq NM_004598.2 (NON-CURRENT)
Match location:
Position 1134 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053594)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGTAATGAGGAGGGCTAT
Hairpin Sequence:
5'-CCGG-CGGTGTAATGAGGAGGGCTAT-CTCGAG-ATAGCCCTCCTCATTACACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGTAATGAGGAGGGCTATCTCGAGATAGCCCTCCTCATTACACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGTAATGAGGAGGGCTATCTCGAGATAGCCCTCCTCATTACACCG-3'

Other clones with same target sequence:

(none)