Construct: shRNA TRCN0000053607

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138403.3-504s1c1
DNA Barcode:
TGAAGGGAAAGGTTTCGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYL10 (93408)
Gene Description:
myosin light chain 10
Transcript:
RefSeq NM_138403.3 (NON-CURRENT)
Match location:
Position 504 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053607)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGGGAAAGGTTTCGTCAA
Hairpin Sequence:
5'-CCGG-TGAAGGGAAAGGTTTCGTCAA-CTCGAG-TTGACGAAACCTTTCCCTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGGGAAAGGTTTCGTCAACTCGAGTTGACGAAACCTTTCCCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGGGAAAGGTTTCGTCAACTCGAGTTGACGAAACCTTTCCCTTCA-3'

Other clones with same target sequence:

(none)