Construct: shRNA TRCN0000053612

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002961.2-225s1c1
DNA Barcode:
AGCTGCTTTCCAGAAGCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
S100A4 (6275)
Gene Description:
S100 calcium binding protein A4
Transcript:
RefSeq NM_002961.2 (NON-CURRENT)
Match location:
Position 225 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053612)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTGCTTTCCAGAAGCTGAT
Hairpin Sequence:
5'-CCGG-AGCTGCTTTCCAGAAGCTGAT-CTCGAG-ATCAGCTTCTGGAAAGCAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTGCTTTCCAGAAGCTGATCTCGAGATCAGCTTCTGGAAAGCAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTGCTTTCCAGAAGCTGATCTCGAGATCAGCTTCTGGAAAGCAGCT-3'

Other clones with same target sequence:

(none)