Construct: shRNA TRCN0000053621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016950.1-1326s1c1
DNA Barcode:
AGGATGATGAAGACGATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPOCK3 (50859)
Gene Description:
SPARC (osteonectin), cwcv and kazal like domains proteoglycan 3
Transcript:
RefSeq NM_016950.1 (NON-CURRENT)
Match location:
Position 1326 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGATGATGAAGACGATATTA
Hairpin Sequence:
5'-CCGG-AGGATGATGAAGACGATATTA-CTCGAG-TAATATCGTCTTCATCATCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGATGATGAAGACGATATTACTCGAGTAATATCGTCTTCATCATCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGATGATGAAGACGATATTACTCGAGTAATATCGTCTTCATCATCCT-3'

Other clones with same target sequence:

(none)