Construct: shRNA TRCN0000053645

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_029962.4-2471s1c1
DNA Barcode:
CGGGTTCAAGAACAAGCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNT1 (57582)
Gene Description:
potassium sodium-activated channel subfamily T member 1
Transcript:
RefSeq XM_029962.4 (NON-CURRENT)
Match location:
Position 2471 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053645)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGTTCAAGAACAAGCTGAT
Hairpin Sequence:
5'-CCGG-CGGGTTCAAGAACAAGCTGAT-CTCGAG-ATCAGCTTGTTCTTGAACCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGTTCAAGAACAAGCTGATCTCGAGATCAGCTTGTTCTTGAACCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGTTCAAGAACAAGCTGATCTCGAGATCAGCTTGTTCTTGAACCCG-3'

Other clones with same target sequence:

(none)