Construct: shRNA TRCN0000053646
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_029962.4-3594s1c1
- DNA Barcode:
- GCAAATTTAACAGCCAGTGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KCNT1 (57582)
- Gene Description:
- potassium sodium-activated channel subfamily T member 1
- Transcript:
- RefSeq XM_029962.4 (NON-CURRENT)
- Match location:
- Position 3594 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000053646)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAAATTTAACAGCCAGTGAT
- Hairpin Sequence:
-
5'-CCGG-GCAAATTTAACAGCCAGTGAT-CTCGAG-ATCACTGGCTGTTAAATTTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAAATTTAACAGCCAGTGATCTCGAGATCACTGGCTGTTAAATTTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAAATTTAACAGCCAGTGATCTCGAGATCACTGGCTGTTAAATTTGC-3'
Other clones with same target sequence:
(none)
