Construct: shRNA TRCN0000053658

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016215.3-716s1c1
DNA Barcode:
GCCAGTCAGATGTGGATGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EGFL7 (51162)
Gene Description:
EGF like domain multiple 7
Transcript:
RefSeq NM_016215.3 (NON-CURRENT)
Match location:
Position 716 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053658)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGTCAGATGTGGATGAAT
Hairpin Sequence:
5'-CCGG-GCCAGTCAGATGTGGATGAAT-CTCGAG-ATTCATCCACATCTGACTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGTCAGATGTGGATGAATCTCGAGATTCATCCACATCTGACTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGTCAGATGTGGATGAATCTCGAGATTCATCCACATCTGACTGGC-3'

Other clones with same target sequence:

(none)