Construct: shRNA TRCN0000053777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002964.3-178s1c1
DNA Barcode:
GTGTCCTCAGTATATCAGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
S100A8 (6279)
Gene Description:
S100 calcium binding protein A8
Transcript:
RefSeq NM_002964.3 (NON-CURRENT)
Match location:
Position 178 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTCCTCAGTATATCAGGAA
Hairpin Sequence:
5'-CCGG-GTGTCCTCAGTATATCAGGAA-CTCGAG-TTCCTGATATACTGAGGACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTCCTCAGTATATCAGGAACTCGAGTTCCTGATATACTGAGGACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTCCTCAGTATATCAGGAACTCGAGTTCCTGATATACTGAGGACAC-3'

Other clones with same target sequence:

(none)