Construct: shRNA TRCN0000053912

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001390.3-1538s1c1
DNA Barcode:
GATGAAGAACACAGGCTAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DTNA (1837)
Gene Description:
dystrobrevin alpha
Transcript:
RefSeq NM_001390.3 (NON-CURRENT)
Match location:
Position 1538 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053912)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAAGAACACAGGCTAATT
Hairpin Sequence:
5'-CCGG-GATGAAGAACACAGGCTAATT-CTCGAG-AATTAGCCTGTGTTCTTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAAGAACACAGGCTAATTCTCGAGAATTAGCCTGTGTTCTTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAAGAACACAGGCTAATTCTCGAGAATTAGCCTGTGTTCTTCATC-3'

Other clones with same target sequence:

  1. TRCN0000301124