Construct: shRNA TRCN0000053944

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153214.1-1186s1c1
DNA Barcode:
CGGCAATGTGAGCTACGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBLN7 (129804)
Gene Description:
fibulin 7
Transcript:
RefSeq NM_153214.1 (NON-CURRENT)
Match location:
Position 1186 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053944)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCAATGTGAGCTACGTGAA
Hairpin Sequence:
5'-CCGG-CGGCAATGTGAGCTACGTGAA-CTCGAG-TTCACGTAGCTCACATTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCAATGTGAGCTACGTGAACTCGAGTTCACGTAGCTCACATTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCAATGTGAGCTACGTGAACTCGAGTTCACGTAGCTCACATTGCCG-3'

Other clones with same target sequence:

  1. TRCN0000174242