Construct: shRNA TRCN0000053978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001792.2-2138s1c1
DNA Barcode:
CCAGTGACTATTAAGAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDH2 (1000)
Gene Description:
cadherin 2
Transcript:
RefSeq NM_001792.2 (NON-CURRENT)
Match location:
Position 2138 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000053978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGACTATTAAGAGAAAT
Hairpin Sequence:
5'-CCGG-CCAGTGACTATTAAGAGAAAT-CTCGAG-ATTTCTCTTAATAGTCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGACTATTAAGAGAAATCTCGAGATTTCTCTTAATAGTCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGACTATTAAGAGAAATCTCGAGATTTCTCTTAATAGTCACTGG-3'

Other clones with same target sequence:

  1. TRCN0000327707