Construct: shRNA TRCN0000054006

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000258.1-345s1c1
DNA Barcode:
CCAAGACAGGAAGAGCTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYL3 (4634)
Gene Description:
myosin light chain 3
Transcript:
RefSeq NM_000258.1 (NON-CURRENT)
Match location:
Position 345 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000054006)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGACAGGAAGAGCTCAAT
Hairpin Sequence:
5'-CCGG-CCAAGACAGGAAGAGCTCAAT-CTCGAG-ATTGAGCTCTTCCTGTCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGACAGGAAGAGCTCAATCTCGAGATTGAGCTCTTCCTGTCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGACAGGAAGAGCTCAATCTCGAGATTGAGCTCTTCCTGTCTTGG-3'

Other clones with same target sequence:

(none)